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All Journal HAYATI Journal of Biosciences Jurnal Ilmu Pertanian Indonesia Jurnal Silvikultur Tropika (JST) Buletin Hama dan Penyakit Tumbuhan Agrotrop : Journal on Agriculture Science Jurnal Natur Indonesia Cropsaver : Journal of Plant Protection Jurnal Penelitian Tanaman Industri BERITA BIOLOGI ZOO INDONESIA JURNAL HAMA DAN PENYAKIT TUMBUHAN TROPIKA Jurnal Penelitian Pertanian Tanaman Pangan Biosaintifika: Journal of Biology & Biology Education JURNAL AGRONIDA Jurnal Entomologi Indonesia Jurnal Penelitian Tanaman Industri (Littri) Buletin Palma Zoo Indonesia
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Departemen Proteksi Tanaman, Fakultas Pertanian, Institut Pertanian Bogor
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Earth & Planetary Sciences Energy Environmental Science Industrial & Manufacturing Engineering Social Sciences Other

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Journal : Jurnal Penelitian Pertanian Tanaman Pangan

 1 
Deteksi Virus Tungro pada Gulma Padi Sawah Menggunakan Teknik PCR Ladja, Fausiah T.; Hidayat, Sri Hendrastuti; Damayanti, Tri Asmira; Rauf, Aunu
Jurnal Penelitian Pertanian Tanaman Pangan Vol 35, No 1 (2016): April 2016
Publisher : Pusat Penelitian dan Pengembangan Tanaman Pangan

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (107.393 KB) | DOI: 10.21082/jpptp.v35n1.2016.p39-44

Abstract

Virus tungro disease is a serious problem to rice crop in a certain area of rice production in Indonesia. The disease is caused by a combined infection of Rice Tungro Bacilliform Virus (RTBV) and Rice Tungro Spherical Virus (RTSV). Both viruses were reported to infect ratoon rice plants, weeds, and wild rice. The study was conducted to detect RTBV and RTSV on some weeds. Weed samples were collected from rice fields in West Java, Bali, West Nusa Tenggara, Papua, and West Sumatera. The detection of RTBV and RTSV were carried out using Polymerase Chain Reaction (PCR) and Reverse Transcription (RT) – PCR, employing coat protein gene specific primers. RTBV specific DNA fragment of ~1400 bp size was successfully amplified from various weed species including: F. miliacea, C. iria, M. vaginalis, L. adscendens, S. zeylanica, D. sanguinalis, and E. crusgalli. RTSV specific DNA fragment of ~787 bp size was successfully amplified from weed species of F. miliacea, L. octovalvis, and D. sanguinalis. RTBV or RTSV specific DNA fragment was not amplified from L. flava and P. distichum. Weed samples infected by both viruses did not show any tungro symptom. Virus detection based on molecular technique was able to determine the status of weed whether it is as an alternate host of viruses. Weeds sanitation prior to rice planting, therefore, should be considered as an integral part of virus disease management.
Co-Authors . Supramana A.A. Ketut Agung Cahyawan W Adriani, Evie AGUSMAN JAYA Ahmad Sifa ALI NURMANSYAH Anwar, Ruli Budi Abduchalek, Budi Dadan Hidayana Dadan Hindayana DADANG DADANG Dede Maryana Dewi Sartiami DJOKO PRIJONO Eka Wahyuningsih ELNA KARMAWATI Evie Adriani GILLIAN W. WATSON GREGORY ALLYN EVANS Harwanto Harwanto Hendri Hermawan Herawani, Febrina Hermanu Triwidodo Husni dan Zulfanazli Pudjianto Alfian Rusdy I Nyoman Widiarta I WAYAN LABA I WAYAN LABA Idham Sakti Harahap IM TRISAWA Indah Putri Januar Yustia Juwita Suri Maharani Kusumah, Yayi Munara Ladja, Fausiah T. LISDAR I. SUDIRMAN Lufthi Rusniarsyah M. SOEHARDJAN M. SOEHARDJAN M. SOEHARDJAN Mahindra Dewi Nur Aisyah MARIETJE PESIRERON Marietje Pesireron Mega wati, Mega Muhammad Zainal Fanani NATALIA VON ELLENRIEDER Nelly Mastina Gultom Nia Kurniawaty Nia Kurniawaty, Nia Nila Wardani Noor Farikhah Haneda NURARIATY AGUS OLUFEMI JOSEPH ALABI Purnama Hidayat PURNAMA HIDAYAT Purnomo Purnomo Qurrotu A’yunin RANGASWAMY MUNIAPPAN Rani Dessy Karyani RAYAPATI ADIKESAVULU NAIDU Rein Estefanus Senewe RIKA RAFFIUDIN Ruly Anwar Rusli Rustam Rusli Rustam Ruth Martha Winnie Samsudin . Samsudin Samsudin Samsudin Samsudin Senewe, Rein Estefanus Senewe, Rein Estefanus Sih Kahono Siswandi Siswandi SITI HERLINDA Siti Masyitah Soemartono Sosromarsono Soemartono Sosromarsono SRI HENDRASTUTI HIDAYAT Sri Utami Sugeng Santoso Sugeng Santoso Tamrin Abdullah Teguh Santoso Teguh Santoso TRI ASMIRA DAMAYANTI Trizelia . UTOMO KARTOSUWONDO UTOMO KARTOSUWONDO UTOMO KARTOSUWONDO Utomo Kartosuwondo Vani Nur Oktaviany Subagyo Wayan Winasa Widi Rumini Yani Maharani Yani Maharani Yani Maharani, Yani YULIA PUJIASTUTI Yuni Maharani